Human amnion epithelial cells: targeted protein profiling using Olink

These datasets contain data from quantification of proteins secreted by human amnion epithelial cells (AECs). Proteins were quantified using Olink's Target 96 Inflammation panel. Data are presented as normalized protein expression (NPX).
Olink analysis 1: contains data from paired samples from 4 placenta donors on AEC-conditioned medium (AEC-CM) from 3 and 7 days of normoxic and hypoxic culture
Olink analysis 2: contains data on AEC-CM from 22 placenta donors, plus a medium control samples (cell culture medium not exposed to AECs).

For more info on analyzed samples, their analysis and our study setup, please see the publication below.

Zeijlon L, Budhwar S, Lindau R, Bencina S, Kaipe H, Jenmalm MC, Gramignoli R and Raffetseder J (2026). Human amnion epithelial cells induce M2 macrophage polarisation partially via M-CSF secretion but independently of extracellular vesicles in vitro. Front. Immunol. 17:1723968. doi: 10.3389/fimmu.2026.1723968
https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2026.1723968/

Abstract:
Pregnancy requires major immunomodulatory changes, both systemically and locally, as the maternal immune system needs to be modulated to tolerate the semi-allogeneic foetus. Decidual macrophages and stromal cells, but also foetal tissues are involved in this immune tolerance, for example by inducing M2 macrophages and regulatory T cells. However, it is so far unknown whether foetal membrane cells such as amnion epithelial cells (AECs) can influence human macrophage polarisation. In this study, a human in vitro macrophage assay was employed to demonstrate that conditioned medium (CM) from AECs derived from term placentas induces M2 macrophage polarisation, and to compare AEC culture conditions aiming for efficient M2 polarisation. Macrophage colony-stimulating factor (M-CSF), a well-known M2-inducing cytokine, was found to be secreted by AECs, and M-CSF was partly responsible for the observed M2-polarising effect of AECs. In addition, the M2-polarising effect remained after removal of extracellular vesicles (EVs) from AEC-CM, suggesting the involvement of soluble but not of EV-associated mediators. Taken together, this study shows that AECs may contribute to the induction of the vital immunotolerant environment at the foetal-maternal interface. Based on their immunomodulatory effects observed here and in in vivo studies, AECs could be harnessed as cytotherapeutics for inflammatory disorders.

本数据集包含人羊膜上皮细胞（human amnion epithelial cells, AECs）分泌蛋白的定量数据。采用Olink Target 96炎症检测面板完成蛋白定量，数据以标准化蛋白表达（normalized protein expression, NPX）形式呈现。
Olink分析1：包含来自4名胎盘供体的配对样本数据，对应常氧及低氧培养3天、7天的AEC条件培养基（AEC-conditioned medium, AEC-CM）。
Olink分析2：包含22名胎盘供体的AEC-CM数据，以及未接触AEC的细胞培养基对照样本。

如需了解分析样本、分析流程及本研究设计的更多细节，请参阅以下发表文献。

Zeijlon L、Budhwar S、Lindau R、Bencina S、Kaipe H、Jenmalm MC、Gramignoli R 及 Raffetseder J (2026). 人羊膜上皮细胞体外通过M-CSF分泌诱导M2巨噬细胞极化且不依赖细胞外囊泡. 《前沿免疫学》(Front. Immunol.) 17:1723968. DOI: 10.3389/fimmu.2026.1723968. 链接：https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2026.1723968/

摘要：妊娠需要全身及局部的重大免疫调节改变，因母体免疫系统需被调控以耐受半同种异体胎儿。蜕膜巨噬细胞、基质细胞及胎儿组织均参与该免疫耐受过程，例如通过诱导M2巨噬细胞与调节性T细胞。然而，目前尚不明确羊膜上皮细胞（AECs）这类胎膜细胞是否可影响人巨噬细胞极化。本研究采用体外人巨噬细胞实验模型，证实源自足月胎盘的AEC条件培养基（conditioned medium, CM）可诱导M2巨噬细胞极化，并旨在优化可高效诱导M2极化的AEC培养条件。研究发现，巨噬细胞集落刺激因子（macrophage colony-stimulating factor, M-CSF）——一种经典的M2诱导细胞因子——可由AECs分泌，且M-CSF是介导AECs诱导M2极化效应的部分原因。此外，将AEC-CM中的细胞外囊泡（extracellular vesicles, EVs）去除后，其M2极化效应仍可维持，提示该效应由可溶性介质而非囊泡相关介质所介导。综上，本研究表明AECs可助力构建胎儿-母体界面关键的免疫耐受微环境。基于本研究及体内研究中观察到的免疫调节效应，AECs有望作为炎症性疾病的细胞治疗手段。