RNA-seq profiling of tubulointerstitial tissue reveals a potential therapeutic role of dual anti-phosphatase 1 in kidney diseases. RNA-seq profiling of tubulointerstitial tissue reveals a potential therapeutic role of dual anti-phosphatase 1 in kidney diseases

We profiled manually microdissected tubulointerstitial tissue from 43 IgA nephropathy, 3 diabetes mellitus nephropathy, 3 focal segmental glomerulosclerosis, 3 lupus nephritis, 4 membranous nephropathy, and 9 minimal change disease biopsy cores and 22 nephrectomy controls by RNA sequencing. The 3 outliers which were not included in our main analysis were also uploaded in this database. Overall design: RNA-seq profiling for manulaly microdissected tubulointerstitial tissue from 90 kidney samples.

本研究通过RNA测序（RNA sequencing），对43例IgA肾病（IgA nephropathy）、3例糖尿病肾病（diabetes mellitus nephropathy）、3例局灶节段性肾小球硬化（focal segmental glomerulosclerosis）、3例狼疮性肾炎（lupus nephritis）、4例膜性肾病（membranous nephropathy）、9例微小病变肾病（minimal change disease）的活检核心组织，以及22例肾切除对照样本的手动显微切割肾小管间质组织完成了转录组表达谱分析。本研究未纳入主分析的3个异常样本，亦上传至本数据库。整体实验设计：针对90份肾脏样本的手动显微切割肾小管间质组织开展RNA-seq表达谱分析。