METTL3-m6A methylation inhibits Type II Alveolar Epithelial Cells proliferation and viability by promoting Pten mRNA stability and translation efficiency in the acute lung injury

In this dataset, we include the m6A methylated RNA data obtained from normal AECII (NC group) and AECII transfected with shMettl3 (T group). We generated the following comparisons using Fold-change (FC) ≥ 2 or ≤ 0.5, and p < 0.05 were used to identify the differentially m6A methylated RNAs.The total RNAs were immunoprecipitated with anti-N6-methyadenosine (m6A) antibody. The modified RNAs were eluted from the immunoprecipitated magnetic beads as the “IP”. The unmodified RNAs were recovered from the supernatant as “Sup”. Two channel arrays were used for these samples but the data were analyzed as single-channel so that each sample represents either IP or supernatent and the sample table includes the normalized signal values. Pleaes note that each raw data file contains both IP and SUP raw data and is linked to the corresponding IP sample records.

本数据集涵盖了来自正常AECII（NC组）以及转染shMettl3的AECII（T组）的N6-甲基腺嘌呤（m6A）甲基化RNA数据。我们以倍数变化（Fold-change, FC）≥2或≤0.5且p值<0.05为筛选标准，鉴定差异m6A甲基化RNA。我们使用抗N6-甲基腺嘌呤（m6A）抗体对总RNA进行免疫沉淀：从免疫沉淀磁珠上洗脱得到的修饰RNA记为“IP组分”，从上清液中回收的未修饰RNA记为“Sup组分”。本数据集的样本采用双通道芯片进行检测，但后续数据分析以单通道模式开展，因此每个样本仅对应IP或Sup组分，样本表中包含标准化后的信号值。请注意，每个原始数据文件均包含IP与SUP的原始数据，并与对应的IP样本记录相关联。