Genome-wide ChIP sequencing of NUCKS. Mus musculus

NUCKS has a DNA binding domain at C-terminal region. We found that NUCKS has important roles in regulating glucose homeostasis. To get an unbiased handle on the plausible mechanism(s) of NUCKS action, we performed a genome-wide Chromatin Immunoprecipitation (ChIP)-sequencing for NUCKS in primary hepatocytes. We successfully mapped 25mln reads to the mm9 genome and detected NUCKS binding at 10203 sites, 60% of which were located in the proximity of a Transcription Start Sites (TSS). The peaks of NUCKS occupancy were often broad with some around 1Kb, suggesting that multiple NUCKS molecules could bind cooperatively to the same genomic loci. This study provides information of NUCKS binding sites in Mus musculus genome. Overall design: Examination of NUCKS binding in mouse primary hepatocytes by Chromatin immunoprecipitation followed by deep sequencing.

NUCKS在其羧基端区域（C-terminal region）携带DNA结合结构域。本研究发现NUCKS在调控葡萄糖稳态过程中发挥关键作用。为无偏探究NUCKS的潜在作用机制，我们在原代肝细胞中针对NUCKS开展了全基因组染色质免疫共沉淀（Chromatin Immunoprecipitation, ChIP）测序。我们成功将2500万条测序读段（reads）比对至mm9小鼠参考基因组，并在10203个基因组位点检测到NUCKS的结合，其中60%的结合位点位于转录起始位点（Transcription Start Sites, TSS）附近。NUCKS的结合占据峰通常较为宽泛，部分峰跨度约1kb，提示多个NUCKS分子可协同结合至同一基因组位点。本研究提供了小家鼠（Mus musculus）基因组中NUCKS结合位点的相关数据。整体实验设计：采用染色质免疫共沉淀联合深度测序的方法，检测小鼠原代肝细胞中NUCKS的结合情况。