Genome Wide DNA Methylation in the Neuronal Fraction of the Prefrontal Cortex from Depressed, Suicide, and Normal Control Subjects

We analyzed the levels of DNA methylation in DNA purified from BA9 of 21 depressed suicide completers and 21 healthy controls, using the Infinium® MethylationEPICBeadChip. The goal of the study was to determine which genes were differentially methylated in neurons between the two groups. We identified1,236 differentially methylated CpGs with a p ≤ 0.001. Gene Ontology and Ingenuity Pathway Analysis were performed to determine classes of genes and pathways associated with the suicide completers. Compare CpG methylation levels in DNA isolated from neurons of the PFC (BA9) of 21 adult depressed suicide completers and 21 healthy control subjects

本研究采用Infinium® 甲基化EPIC微珠芯片（Infinium® MethylationEPIC BeadChip），对21例抑郁自杀死亡者与21例健康对照者的布罗德曼9区（Brodmann Area 9，BA9）提取的纯化DNA开展DNA甲基化水平分析。本研究的核心目标为明确两组受试者神经元中存在甲基化差异的基因。本研究共鉴定出1236个甲基化差异CpG位点（CpG），显著性阈值为p≤0.001。我们通过基因本体（Gene Ontology, GO）富集分析与Ingenuity通路分析（Ingenuity Pathway Analysis, IPA），筛选出与自杀死亡者相关的基因类别及信号通路。本研究同时对21例成年抑郁自杀死亡者及21例健康对照者的前额叶皮层（Prefrontal Cortex, PFC，BA9）神经元提取的DNA开展CpG甲基化水平比较。