Hela细胞

细胞来源：人；细胞种类：子宫颈癌；生长状态：贴壁；建议培养基：MEM+10%牛血清+抗生素 对于贴壁细胞应先吸（倒）尽培养基，以免中和后加入的消化液，使强度减弱（或PBS洗1－3次）。50ml培养瓶加入消化液约1-3ml，按此比例进行消化，晃动使消化液铺均匀置37度培养箱约2-5分钟，镜下见细胞收缩变圆或少数脱落后，轻轻振动瓶底使细胞全部脱落，加入2-3ml完全培养基后，轻轻吹打，使细胞基本成单个悬浮，然后分置其它无菌培养瓶内，加入完全培养基后继续培养或实验。悬浮细胞:一般传代可直接将细胞原液分置其它培养瓶内，加入完全培养基继续培养，如要高浓度可先离心1000rpm，5min后加入完全培养基，轻轻吹匀后，分置其它培养瓶内加入完全培养基继续培养。

Cell Source: Human; Cell Type: Cervical Cancer; Growth State: Adherent; Recommended Culture Medium: MEM + 10% Bovine Serum + Antibiotics. For adherent cells, first aspirate (decant) the culture medium completely to avoid neutralizing the subsequently added digestive solution and reducing its efficacy, or wash the cells with PBS for 1 to 3 times. Add approximately 1-3 mL of digestive solution to a 50 mL culture flask, and perform digestion according to this ratio. Gently shake the flask to evenly distribute the digestive solution, then place it in a 37°C incubator for about 2 to 5 minutes. When observed under a microscope, if the cells have shrunk and rounded up or a small portion has detached, gently tap the bottom of the flask to detach all cells. Add 2-3 mL of complete culture medium, then gently pipette to dissociate the cells into mostly single-cell suspensions. Next, aliquot the cell suspension into other sterile culture flasks, add complete culture medium, and continue culturing or proceed with experiments. For suspension cells: For routine passaging, directly aliquot the original cell culture into other culture flasks, add complete culture medium, and continue culturing. If a higher cell concentration is required, first centrifuge the culture at 1000 rpm for 5 minutes, resuspend the cell pellet in complete culture medium, gently pipette to mix evenly, then aliquot into other culture flasks, add complete culture medium, and continue culturing.