Real-time quantitative PCR detection of Ts-Pt mRNA at different developmental stages of T. spiralis.
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The relative expression of Ts-Pt mRNA was evaluated using the 2−ΔCt method. GAPDH was used as an internal control. There were statistically significant differences (p
采用2−ΔCt法对Ts-Pt信使核糖核酸(mRNA)的相对表达量进行测定,以甘油醛-3-磷酸脱氢酶(GAPDH)作为内参基因。各组间存在统计学显著性差异(p
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2016-02-23



