Comparative proteomic analysis between light and dark-adapted Euglena gracilis

We sequenced and assembled a detailed transcriptome and draft genome for E. gracilis Z1. To improve annotation and investigate gene expression in E. gracilis, where most transcripts are also trans-spliced, we conducted comparative proteomic and transcriptomic analysis between light and dark-adapted E. gracilis. The analysis revealed that alterations to protein abundance are controlled post-transcriptionally, surprisingly similar to trypanosomatids.

我们对纤细裸藻（E. gracilis）Z1进行了测序，并组装得到其精细转录组与草图基因组。为优化该物种的基因注释并探究其基因表达模式（该物种的大多数转录本均存在反式剪接现象），我们对光适应与暗适应状态下的纤细裸藻开展了比较蛋白质组与转录组联合分析。分析结果显示，蛋白质丰度的变化受转录后水平调控，这一特征与锥虫科原虫的情况出乎意料地相似。