Elevated atmospheric CO2 concentration decreases prokaryotic diversity in the rhizosphere of barley (Hordeum vulgare L.). Barley rhizosphere 16S

Barley (Hordeum vulgare L.) was grown under ambient (400 ppm) or elevated (700 ppm) atmospheric CO2 concentration in a field experiment using open-top chambers. The root associated microbiota of the plants were sampled and the samples subjected to PCR targeting the V4 region of the 16S rRNA gene using primers S-D-Arch-0519-a-S-15 and S-D-Bact-0785-a-A-21. PCR products were sequenced on an Illumina MiSeq instrument with 500 cycle v2 kit to obtain 251 bp long paired-end reads.

本田间试验采用开顶箱（open-top chambers）开展，将大麦（Hordeum vulgare L.）分别种植于大气CO₂浓度为环境水平（400 ppm）与升高水平（700 ppm）的条件下。采集植株的根系相关微生物群落样品，使用引物S-D-Arch-0519-a-S-15与S-D-Bact-0785-a-A-21，针对16S核糖体RNA（16S rRNA）基因的V4区对样品进行聚合酶链式反应（PCR）扩增。扩增产物采用搭配500循环v2试剂盒的Illumina MiSeq测序仪进行测序，获得长度为251 bp的双端读段（paired-end reads）。