Supplemental Information for A new transcriptome and transcriptome profiling of adult and larval tissue in the box jellyfish Alatina alata: an emerging modelfor studying venom, vision and sex.

Cubozoans (box jellyfish) are cnidarians<b> </b>that have evolved a number of distinguishing features. Many cubozoans have a particularly potent sting, effected by stinging structures called nematocysts; cubozoans have well-developed light sensation, possessing both image-forming lens eyes and light-sensitive eye spots; and some cubozoans have complex mating behaviors, including aggregations, copulation and internal fertilization. The cubozoan <i>Alatina alata</i> is emerging as a cnidarian model because it forms predictable monthly nearshore breeding aggregations in tropical to subtropical waters worldwide, making both adult and larval material reliably accessible. To develop resources for <i>A. alata</i>, this study generated a functionally annotated transcriptome of adult and larval tissue, applying preliminary differential expression analyses to identify candidate genes involved in nematogenesis and venom production, vision and extraocular sensory perception, and sexual reproduction, which for brevity we refer to as “venom”, “vision” and “sex”.

立方水母（Cubozoans, box jellyfish）隶属于刺胞动物（cnidarians），现已演化出诸多标志性特征。多数立方水母拥有极强的刺蛰能力，该功能由名为刺细胞（nematocysts）的特化结构完成；立方水母具备高度发达的光感知系统，同时兼具成像透镜眼与感光眼斑；部分类群还拥有复杂的交配行为，包括群体聚集、交配过程与体内受精。*Alatina alata*正逐渐成为刺胞动物研究的模式物种，因其可在全球热带至亚热带海域的近岸区域形成可预测的月度繁殖群体，使得成体与幼体样本均可稳定获取。为构建*Alatina alata*的研究资源，本研究生成了成体与幼体组织的功能注释转录组（transcriptome），并通过初步的差异表达分析筛选出与刺细胞发生（nematogenesis）、毒液产生、视觉及眼外感官感知、有性生殖相关的候选基因；为简洁起见，我们将这几类基因分别简称为“毒液”“视觉”与“性”相关基因。