Genomics polymorphisms of Staphylococcus aureus strain NCTC 8325 in the lab stock maintained at TUM (WT), after 30 passes in BHI media (D) and after 30 passes detecting 4 -fold MIC increase to isocyanide -code I16- 3 biological replicates (A,B,C), and 3 independent colonies sequenced per replicate at the end of the experiment.

Genomics polymorphisms of Staphylococcus aureus strain NCTC 8325 in the lab stock maintained at TUM (WT), after 30 passes in BHI media (D) and after 30 passes detecting 4 -fold MIC increase to isocyanide -code I16-  3 biological replicates (A,B,C), and 3 independent colonies sequenced per replicate at the end of the experiment. Determined from Illumina shotgun genomic sequencing datasets, mapping and analyses vs the reference genome of the strain https://www.ncbi.nlm.nih.gov/nuccore/NC_007795.1/

本数据集针对保藏于慕尼黑工业大学（Technische Universität München, TUM）实验室菌种库的金黄色葡萄球菌（Staphylococcus aureus）菌株NCTC 8325的基因组多态性展开分析，包含三类实验样本：一是野生型（Wild Type, WT）原始保藏菌株；二是在脑心浸液肉汤（Brain Heart Infusion, BHI）培养基中连续传代30次后的菌株（标记为D）；三是经30次传代后对异氰化物类化合物I16的最低抑菌浓度（Minimum Inhibitory Concentration, MIC）提升4倍的菌株。该实验组设置3个生物学重复（分别标记为A、B、C），实验结束后每个重复选取3个独立菌落进行测序。所有测序数据均通过Illumina鸟枪基因组测序技术获取，并以该菌株的参考基因组（https://www.ncbi.nlm.nih.gov/nuccore/NC_007795.1/）为参照进行序列比对与分析。