2-aminoimidazoles potentiate ß-lactam antimicrobial activity against Mycobacterium tuberculosis by reducing ß-lactamase secretion and increasing cell wall permeability

The purpose of this study was to examine the impact of 2-aminoimidazoles on M. tuberculosis transcriptional responese. M. tuberculosis H37Rv was cultured in 7H9 media to an OD600 of 0.4 then treated with 125 µM 2B8 for 2 and 24 h. Following treatment, RNA was isolated from the bacterial cultures and used for RNA-seq.

本研究旨在探究2-氨基咪唑类化合物对结核分枝杆菌（Mycobacterium tuberculosis，缩写M. tuberculosis）转录应答的影响。将结核分枝杆菌H37Rv接种于7H9培养基中培养至600nm光密度（OD600）为0.4，随后以125 μM的2B8分别处理2小时与24小时。处理结束后，从细菌培养物中分离RNA，用于RNA测序（RNA-seq）。