Evaluation of biochemical and electrolytic components of semen from ram supplemented with different concentrations of selenium and its correlation with sperm quality

ABSTRACT The aim of the study was to evaluate whether supplementation with different levels of selenium (Se) can change the biochemical and electrolytic components of semen, causing an improvement in seminal quality in rams. Thirty animals were kept in an intensive pen system, fed with hay and commercial ration, allocated into five groups (six animals/pen) and supplemented with a mineral mixture containing 0mg (G1), 5mg (G2), 10mg (G3), 15mg (G4) and 20mg (G5) of Se/kg. Each group received a different treatment every 56 days and treatments were rotated between groups following a dynamic sequence. Semen samples were collected by electroejaculation after the end of each treatment to evaluate the levels of fructose, citric acid, potassium (K), sodium (Na), calcium (Ca), Se, zinc (Zn), manganese (Mn), sulfur (S) and lead (Pb). The statistical design was a 5x5 Latin square. The different levels of Se supplementation evaluated maintained the concentrations of electrolytes and minerals in the semen at the required levels and did not change the sperm quality, concluding that higher intakes of Se do not cause antagonistic effects on the absorption and subsequent action of other essential minerals supplied to the animals and still maintains electrolyte balance.

摘要：本研究旨在评估补充不同水平硒（Selenium，Se）是否可改变公羊精液的生化与电解质成分，进而改善其精液品质。选取30只采用集约化圈养系统的受试公羊，饲喂干草与商品配合饲料，随机分为5组（每组6只，单圈饲养），各组分别饲喂添加0mg（G1组）、5mg（G2组）、10mg（G3组）、15mg（G4组）及20mg（G5组）硒/kg的矿物混合料。每56天更换一次处理方案，并通过动态轮换机制在各组间循环实施不同处理。每轮处理结束后，采用电刺激采精法采集精液样本，检测其中果糖、柠檬酸、钾（K）、钠（Na）、钙（Ca）、硒（Se）、锌（Zn）、锰（Mn）、硫（S）及铅（Pb）的含量。本研究采用5×5拉丁方试验设计。结果表明，不同水平的硒补充剂可使精液中电解质与矿物质浓度维持在所需范围内，且未对精子品质产生显著影响；由此得出结论：高剂量硒摄入不会对动物所需其他必需矿物质的吸收及后续作用产生拮抗效应，同时可维持精液电解质平衡。