Data showing positive regulation of Wnt/β-catenin signal transduction by FAM129B siRNA using transcriptional reporter assay, target gene expression, and apoptosis assay

Data for Figure 2b. Measured β-catenin dependent firefly: constitutive renilla ratios for HT1080, A375, and A2058 reporter cells treated with the indicated conditions. Data for Figure 2c: Quantitative RT-PCR of β-catenin dependent Axin2 mRNA and housekeeping actin mRNA for HT1080, A375, and A2058 cells treated with the indicated conditions. Data for Figure 3a. Measured β-catenin dependent firefly: constitutive renilla ratios for A375 reporter cells treated with a dose curve of WNT3A or a single dose of WNT3A. Data for Figure 3b. Measured NFκB dependent firefly: constitutive renilla ratios for A375 reporter cells treated with a dose curve of TNFalpha or a single dose of TNFα . Data for Figure 4b. Quantitation of DAPI and TUNEL positive nuclei from A375 cells treated with WNT3A, PLX4720, and the control, FAM129B, or CTNNB1 siRNA.

图2b对应数据：经指定条件处理的HT1080、A375及A2058报告细胞中，β-连环蛋白(β-catenin)依赖性萤火虫荧光素酶与组成型表达海肾荧光素酶的活性比值检测结果。
图2c对应数据：经指定条件处理的HT1080、A375及A2058细胞中，β-连环蛋白依赖性Axin2 mRNA与管家基因肌动蛋白(actin) mRNA的实时定量逆转录聚合酶链反应(Quantitative RT-PCR)检测结果。
图3a对应数据：经WNT3A(WNT3A)剂量梯度或单剂量处理的A375报告细胞中，β-连环蛋白依赖性萤火虫荧光素酶与组成型表达海肾荧光素酶的活性比值检测结果。
图3b对应数据：经肿瘤坏死因子α(TNFα)剂量梯度或单剂量处理的A375报告细胞中，核因子κB(NFκB)依赖性萤火虫荧光素酶与组成型表达海肾荧光素酶的活性比值检测结果。
图4b对应数据：经WNT3A、PLX4720、对照试剂、FAM129B小干扰RNA(siRNA)或CTNNB1小干扰RNA(siRNA)处理的A375细胞中，4',6-二脒基-2-苯基吲哚(DAPI)与脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)阳性细胞核的定量分析结果。