Multimaterial 3D Laser Printing of Cell-Adhesive and Cell-Repellent Hydrogels [data]

This study introduces a straightforward method for manufacturing 3D microstructured cell-adhesive and cell-repellent multimaterials using two-photon laser printing. Compared to existing strategies, this approach offers bottom-up molecular control, high customizability and rapid and precise 3D fabrication. The printable cell-adhesive PEG-based material includes an RGD-containing peptide synthesized through solid-phase peptide synthesis, allowing for precise control of the peptide design. Remarkably, minimal amounts of RGD peptide (< 0.1 wt%) suffice for imparting cell-adhesiveness, while maintaining identical mechanical properties in the 3D printed microstructures to those of the cell-repellent, PEG-based material. Fluorescent labeling of the RGD peptide facilitates visualization of its presence in cell-adhesive areas. To demonstrate the broad applicability of our system, we showcase the fabrication of cell-adhesive 2.5D and 3D structures, fostering the adhesion of fibroblast cells within these architectures. Thus, this approach allows for the printing of high-resolution, true 3D structures suitable for diverse applications, including cellular studies in complex environments.

这项研究介绍了一种利用双光子激光打印（two-photon laser printing）制备三维微结构化细胞黏附与细胞排斥多材料的简便方法。与现有策略相比，该方法具有自下而上的分子调控能力、高度可定制性以及快速精准的三维制造特性。可打印的细胞黏附聚乙二醇（PEG）基材料包含通过固相肽合成（solid-phase peptide synthesis）制备的含RGD肽段，能够实现肽段设计的精准控制。值得注意的是，极少量的RGD肽段（<0.1 wt%）即可赋予材料细胞黏附性，同时使三维打印微结构的力学性能与细胞排斥性PEG基材料保持一致。对RGD肽段进行荧光标记可便于观察其在细胞黏附区域的存在。为展示该系统的广泛适用性，我们制备了细胞黏附性2.5D及3D结构，促进成纤维细胞在这些结构中的黏附。因此，该方法可打印高分辨率的真正三维结构，适用于多种应用场景，包括复杂环境下的细胞研究。