Single-cell RNA sequencing of 6 follicular lymphoma tumors

Follicular lymphoma (FL) is an indolent cancer of mature B-cells but carries increased risk of transformation to a more aggressive histology over time. We present here comprehensive profiling both tumor and immune compartments in 6 diagnostic FL biopsies by single-cell RNA sequencing. This confirmed results from 155 FL tumors characterized by mass cytometry (CyTOF) which revealed two distinct evolutionary trajectories with disparate risk of transformation and alternate biologies.EGA study EGAS00001005257

滤泡性淋巴瘤（Follicular lymphoma, FL）是一类起源于成熟B细胞的惰性恶性肿瘤，随病程进展，其向侵袭性更强的组织学类型转化的风险显著升高。本研究采用单细胞RNA测序（single-cell RNA sequencing）技术，对6例诊断性FL活检样本的肿瘤与免疫微环境组分开展了全面表征分析。该研究结果在155例经质谱流式细胞术（mass cytometry, CyTOF）表征的FL肿瘤样本中得到验证，后者揭示了两条具有迥异转化风险与独特生物学特征的独立进化轨迹。本研究的EGA（European Genome-phenome Archive）编号为EGAS00001005257