Wild type 4 hours exposure to congo red (cell wall chip)

S. cerevisiae was grown on YEPD. For Congo Red experiments, yest cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with Congo Red to a final concentration of 30 µg/ml. Cells were collected at 4hours of growth, frozen at -80 °C and processed for RNA extraction. Keywords: repeat sample

酿酒酵母（S. cerevisiae）于酵母提取物蛋白胨葡萄糖培养基（YEPD）中培养。针对刚果红实验，酵母细胞于24℃下过夜培养至光密度（A600）为0.8~1。将培养液稀释至0.2 OD后，于24℃继续培养2小时。随后将培养液均分为两份：一份在原培养条件下继续培养（即未处理对照组），另一份添加刚果红至终浓度30 μg/mL。培养4小时后收集细胞，于-80℃冻存并进行RNA提取。关键词：重复样本