Whole-genome resequencing of BC1 populations from Arabidopsis (Col x Ler) x Col crosses (wild type and zyp1). Whole-genome resequencing of BC1 populations from Arabidopsis (Col x Ler) x Col crosses (wild type and zyp1)

This experiment aims at analyzing crossover distribution in male and female meiosis, in the Arabidopsis zyp1 mutant compared to wild type. Plants heterozygous for the zyp1-1 mutation (Col) were crossed as female with plants heterozygous for the zyp1-6 mutation (Ler). Wild type and zyp1-1/zyp-6 plants were selected among the F1s, and crossed as male or as female with wild-type Col. Leaf samples from plants from the four obtained BC1 populations were used for DNA purification and library preparation for Illumina sequencing (HiSeq 3000 2x150 bp), performed by the Max Planck-Genome-centre Cologne, Germany (https://mpgc.mpipz.mpg.de/home/).

本实验旨在分析拟南芥（Arabidopsis）zyp1突变体与野生型（wild type）雌雄减数分裂（meiosis）中的交叉分布（crossover distribution）。将携带zyp1-1突变（Col背景）的杂合植株作为母本，与携带zyp1-6突变（Ler背景）的杂合植株进行杂交。从F1代中筛选出野生型与zyp1-1/zyp-6植株，分别作为父本或母本与野生型Col植株杂交。从所得4个BC1回交群体的植株中采集叶片样本，用于DNA纯化（DNA purification）与文库制备（library preparation），随后交由德国科隆马克斯·普朗克基因组中心（Max Planck-Genome-centre Cologne, Germany）完成Illumina测序（Illumina sequencing，采用HiSeq 3000 2×150 bp测序模式），该中心官方网址为https://mpgc.mpipz.mpg.de/home/。