GLI3 binding regions in mouse embryonic forelimb buds

Purpose: The response to Hedgehog signaling in the limb is driven by GLI bound enhancers and the majority of Hh targets in the developing limb bud are regulated solely by the activity of GLI-repressor. Currently we do not have a comprehensive understanding of how GLI bound enhancers respond Hedgehog signaling. The goal of this study is to identify how GLI bound enhancers are regulated by Hedgehog signaling and specifically by GLI-repressor. Methods: ChIP-seq was performed in Embryonic day 10.5 mouse limb buds from mice with endogenously FLAG tagged Gli3. Results: We identified 7282 GLI3 binding regions in the E10.5 limb bud. ChIP-sequencing E10.5 forelimb buds from mice with enogenously FLAG-tagged Gli3. Two biological replicates and one input control were submitted.

研究目标：肢体中的刺猬信号通路（Hedgehog signaling）响应由结合GLI的增强子所介导，发育肢芽中的大多数刺猬信号通路靶基因仅通过GLI阻遏物（GLI-repressor）的活性实现调控。目前我们尚未全面解析结合GLI的增强子如何响应刺猬信号通路。本研究旨在明确结合GLI的增强子如何受刺猬信号通路调控，且具体受GLI阻遏物调控。
实验方法：对携带内源性FLAG标签标记Gli3的小鼠，取其胚胎第10.5天（E10.5）的肢芽开展染色质免疫共沉淀测序（ChIP-seq）；具体实验中，我们对携带内源性FLAG标签标记Gli3的小鼠的E10.5前肢芽进行染色质免疫共沉淀测序，本次实验包含两份生物学重复样本与一份输入对照样本。
实验结果：本研究在E10.5小鼠肢芽中鉴定出7282个GLI3结合区域。