Gene expression profiles of caseous human pulmonary TB granulomas derived from TB patients. Homo sapiens

Most individuals infected with Mycobacterium tuberculosis can control the infection by forming and maintaining TB granulomas at the local infection foci. However, when the chronic infection (also known as latency) becomes active, the caseous center of TB granuloma enlarges, and it liquefies and cavitates, ultimately releasing bacilli into airway. Deciphering how genes are regulated within TB granulomas will help to understand the granuloma biology. Therefore, we performed genome-wide microarray on caseous human pulmonary TB granulomas and compared with normal lung tissues. Overall design: Laser capture microdissection (LCM) was used to dissect out caseous granulomas from TB patients' lung tissues, excluding uninvolved areas. Total RNA were isolated from LCM-derived materials and used for microarray. As a control, parenchyma from normal lung tissues was prepared in the same manner as caseous granulomas. Sample GSM501252, Caseum 2-C, is missing a CEL file.

大多数感染结核分枝杆菌（Mycobacterium tuberculosis）的个体可通过在局部感染灶形成并维持结核肉芽肿（TB granuloma）从而控制感染。然而，当慢性感染（亦称潜伏感染）转为活动期时，结核肉芽肿的干酪样中心会逐渐增大，继而发生液化与空洞形成，最终将结核杆菌释放至气道内。解析结核肉芽肿内的基因调控机制，将有助于阐明肉芽肿的生物学特性。为此，我们针对人类肺部结核肉芽肿的干酪样病灶开展了全基因组微阵列（genome-wide microarray）分析，并以正常肺组织作为对照进行比较。整体实验设计：采用激光捕获显微切割（Laser capture microdissection, LCM）技术从结核患者的肺组织中分离干酪样肉芽肿，同时排除未受累区域。从激光捕获显微切割获取的样本中提取总RNA，用于微阵列实验。作为对照，以相同方法处理正常肺组织的肺实质样本，与干酪样肉芽肿样本保持一致。样本GSM501252（Caseum 2-C）缺失CEL文件。