Surveillance of 3’ mRNA cleavage during transcription termination requires CF IB/Hrp1

Comparison of cytoplasmic 3' elongated transcripts in hrp1-1 and wild type. To invesatigate 3' elongated transcripts in the cytoplasm of the mutant strain hrp1-1 we created a cytoplasmic and total fraction of wild type and hrp1-1 cells. RNA was isolated and sequenced.

本研究对比分析hrp1-1突变株与野生型的细胞质3'端延伸转录本（3' elongated transcripts）。为探究hrp1-1突变菌株细胞质中的3'端延伸转录本，我们分别从野生型与hrp1-1细胞中分离得到细胞质组分与全细胞总组分，随后对提取的RNA进行测序。