Porous matrix of BG60S associated with allogenic osteoprogenitor cells in the treatment of critical bone defects in the radius of dogs

ABSTRACT The objective of this study was to evaluate the effect of the porous matrix of bioglass 60S (BV60S) associated with allogenic osteoprogenitor cells (CO) in the treatment of critical bone defects of dogs. 20 male mongrel dogs at two years old and mean weight of 25kg were used. Dogs were anesthetized and critical bone defects were created in the middle third of the radios bones. With dogs under general anesthesia, critical bone defects were created in the middle third of bone radios. Bone fixation was done with a bridge plate and defects treated according to each experimental group. Three experimental groups were formed according to the treatment. The defects filled with BV60S associated with allogenic CO (Group-BV60S+CO), autogenous bone (Group-C+) or unfilled (Group-C-). Bone regeneration was evaluated by radiography, bone densitometry and histomorphometry over 90 days. The BV60S+CO and C- groups showed partial bone filling of the defect of at most 56.68% and 35.23%, respectively. No bone bridge formation was observed between the extremities in the BV60S+CO and C- groups. Positive control showed complete bone regeneration at 90 days. It was concluded that the porous matrix of BV60S associated with osteoprogenitor cells was not effective in the treatment of critical bone defects in the radius of dogs.

摘要 本研究旨在评估生物玻璃60S（bioglass 60S，BV60S）多孔支架联合同种异体骨祖细胞（allogenic osteoprogenitor cells，CO）治疗犬临界骨缺损的效果。本研究选用20只2岁龄、平均体重25kg的雄性杂种犬，对其实施全身麻醉后于桡骨中1/3段制备临界骨缺损模型，以接骨板完成骨固定，并根据实验组方案对骨缺损进行处理。本研究设置3个实验组：缺损处填充BV60S联合同种异体CO组（Group-BV60S+CO）、自体骨组（Group-C+）以及空白缺损组（Group-C-）。分别于术后90天通过X线摄影、骨密度测定及组织形态计量学评估骨再生情况。结果显示，BV60S+CO组与C-组的缺损骨填充率最高分别为56.68%与35.23%，两组缺损骨断端均未观察到骨桥形成；自体骨组（阳性对照）于术后90天实现完全骨再生。本研究证实，BV60S多孔支架联合骨祖细胞用于治疗犬桡骨临界骨缺损并无显著效果。