Molecular Characterisation of Cold-Adapted Metallo-Oxotransferases from the Dimethylsulfoxide Reductase Family Expressed by Antarctic Bacteria

Metadata record for data from ASAC Project 2547
See the link below for public details on this project.

Pue (greater than 90% as determined by SDS-PAGE) samples of nitrate reductase have been isolated from the Antarctic bacterium, Shewanella gelidimarina (ACAM 456T; Accession number U85907 (16S rDNA)). The protein is ~90 kDa (similar to nitrate reductase enzymes characterised from alternate bacteria) and stains positive in an in-situ nitrate reduction (native) assay technique. The protein may be N-terminal blocked, although further sequencing experiments are required to confirm this.

This work is based upon phenotyped Antarctic bacteria (S. gelidimarina; S.frigidimarina) that was collected during other ASAC projects. (Refer: Psychrophilic Bacteria from Antarctic Sea-ice and Phospholipids of Antarctic sea ice algal communities new sources of PUFA [ASAC_708] and Biodiversity and ecophysiology of Antarctic sea-ice bacteria [ASAC_1012]).

The download file contains 4 scientific papers produced from this work - one of these papers also contains a large set of accession numbers for data stored at GenBank.

ASAC项目2547数据的元数据记录
项目的公开详情请参见下方链接。

纯度（Pue，经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）测定大于90%）的硝酸还原酶样本已从南极细菌Shewanella gelidimarina（ACAM 456T；16S rDNA登录号U85907）中分离得到。该蛋白分子量约为90 kDa（与其他细菌中已表征的硝酸还原酶相似），且在原位硝酸还原（native）测定技术中染色呈阳性。该蛋白可能存在N端封闭，不过需进一步测序实验验证这一点。

这项工作基于其他ASAC项目期间采集的表型鉴定南极细菌（S. gelidimarina；S. frigidaimarina）。（参考：南极海冰嗜冷细菌与南极海冰藻类群落磷脂——PUFA的新来源[ASAC_708]；南极海冰细菌的生物多样性与生理生态学[ASAC_1012]）。

下载文件包含本研究产出的4篇科学论文——其中一篇还包含GenBank中存储数据的大量登录号。