Data_Sheet_1_Genome-wide characterization of cys-tathionine-β-synthase domain-containing proteins in sugarcane reveals their role in defense responses under multiple stressors.ZIP

Cys-tathionine-β-synthase (CBS) domain-containing proteins (CDCPs) are essential for regulating plant responses to various biotic and abiotic stressors. This study describes the systematic identification and characterization of CDCP family genes in Saccharum spontaneum. A total of 95 SsCDCP genes and eight phylogenetic groups were identified that were distributed over 29 chromosomes of the AP85-441 genome. Most (78/95) SsCDCPs underwent fragment duplication events, and 64 gene pairs were located in synteny blocks. Expression profiling of nine ShCDCPs was also carried out in the Saccharum spp. cultivars ROC22 and MT11-611 that are resistant and susceptible to red stripe, respectively, in response to: (i) Infection by the bacterial pathogen Acidovorax avenue subsp. avenae (Aaa); (ii) abiotic stressors (drought and salinity); and (iii) exogenous salicylic acid (SA) treatment. Members of one gene pair (ShCBSD-PB1-5A and ShCBSD-PB1-7A-1) with a fragment duplication event acted as negative regulators in sugarcane under four stresses, as supported by the significantly decreased expression levels of ShCBSD-PB1-5A (23–83%) and ShCBSD-PB1-7A-1 (15–75%) at all-time points, suggesting that they have functional redundancy. Genes in another pair, ShCBS-4C and ShCBS-4D-1, which have a fragment duplication event, play opposing regulatory roles in sugarcane exposed to multiple stresses, particularly Aaa and NaCl treatments. ShCBS-4C expression was significantly decreased by 32–77%, but ShCBS-4D-1 expression was dramatically upregulated by 1.2–6.2-fold in response to Aaa treatment of both cultivars across all-time points. This result suggested that both genes exhibited functional divergence. Meanwhile, the expression of SsCBSDCBS-5A was significantly upregulated in ROC22 by 1.4–4.6-fold in response to the four stressors. These findings provide important clues for further elucidating the function of ShCDCP genes in sugarcane responding to a diverse range of stresses.

含半胱氨酸硫醚β合酶（Cys-tathionine-β-synthase, CBS）结构域的蛋白质（CDCPs）在调控植物应对各类生物与非生物胁迫的响应过程中发挥关键作用。本研究对甜根子草（Saccharum spontaneum）中的CDCP家族基因进行了系统鉴定与表征。研究共鉴定得到95个SsCDCP基因，可划分为8个系统发育类群，这些基因分布于AP85-441基因组的29条染色体上。其中绝大多数（78/95）SsCDCP基因发生了片段复制事件，且有64个基因对位于共线性区块中。本研究还针对两个甘蔗属栽培品种——抗红条病品种ROC22与感病品种MT11-611——中的9个ShCDCP基因开展了表达谱分析，处理条件包括：(i) 细菌病原菌燕麦嗜酸菌燕麦亚种（Acidovorax avenue subsp. avenae, Aaa）侵染；(ii) 非生物胁迫（干旱与盐胁迫）；(iii) 外源水杨酸（salicylic acid, SA）处理。一对发生片段复制事件的基因（ShCBSD-PB1-5A与ShCBSD-PB1-7A-1）在甘蔗遭受四种胁迫时均作为负调控因子发挥作用，对应基因在各时间点的表达量均显著下调：ShCBSD-PB1-5A下调23%~83%，ShCBSD-PB1-7A-1下调15%~75%，提示二者存在功能冗余。另一对发生片段复制事件的基因ShCBS-4C与ShCBS-4D-1则在甘蔗应对多种胁迫时呈现相反的调控模式，尤其在Aaa侵染与NaCl处理下表现显著：在两个栽培品种的各时间点处理中，ShCBS-4C的表达量显著下调32%~77%，而ShCBS-4D-1的表达量则显著上调1.2~6.2倍，该结果表明二者存在功能分化。同时，在ROC22中，SsCBSDCBS-5A的表达量在应对四种胁迫时显著上调1.4~4.6倍。本研究结果为进一步解析甘蔗ShCDCP基因应对多样化胁迫的生物学功能提供了重要线索。