Transcriptional response of soybean plants to soybean mosaic virus infection. Glycine max

Transcriptional changes in soybean plants infected with Soybean mosaic virus (SMV) was assessed at 7, 14 and 21 days post inoculation (dpi). Keywords: Time course Overall design: Two separate cDNA labeling reactions (Cy3 and Cy5), one for the RNA from SMV-infected leaf tissues and the other for the RNA from the mock-inoculated leaves, were carried out. A total of 18 microarray slides were used for this study: 6 array hybridizations including three reciprocal labeling experiments from three independent biological replications of either SMV-infected or mock-inoculated leaf at each of the three time points. Dye swaps were performed to ensure that the results were not biased by dye effects.

本研究检测了接种大豆花叶病毒（Soybean mosaic virus, SMV）的大豆植株在接种后7、14、21天（days post inoculation, dpi）的转录组变化。
关键词：时间进程（Time course）
整体实验设计：本实验开展两组独立的cDNA荧光标记反应（分别采用Cy3与Cy5染料）：一组用于标记SMV侵染叶片组织的RNA，另一组用于标记模拟接种（mock-inoculated）叶片的RNA。本研究共使用18张基因芯片（microarray）玻片，完成6次芯片杂交实验；该6次杂交涵盖3个时间点下，SMV侵染组与模拟接种组叶片样本各3次独立生物学重复的反向标记杂交实验。实验通过设置染料互换（dye swap）对照，规避了荧光染料偏好性对实验结果造成的偏差。