Deletion of the Tug1 locus leads to male sterility and affects gene expression in multiple tissues

We have sequenced wild type and mutant testes, prostate, liver, heart, spleen, and eye from adult males, as well as embryonic fibroblasts from the mutant lncRNA strain Tug1_tm1.1Vlcg (Tug1). Overall design: Comparison between wild type and mutant organs and primary cell transcriptomes from the Tug1_tm1.1Vlcg mutant strain.

本研究对成年雄性个体的野生型与突变型睾丸、前列腺、肝脏、心脏、脾脏及眼部组织，以及突变型长链非编码RNA（long non-coding RNA，lncRNA）菌株Tug1_tm1.1Vlcg（简称Tug1）的胚胎成纤维细胞开展了转录组测序。
实验设计：比较该Tug1_tm1.1Vlcg突变菌株的野生型与突变型器官及原代细胞的转录组。