Additional file 2 of Design and validation of recombinant protein standards for quantitative Western blot analysis of cannabinoid CB1 receptor density in cell membranes: an alternative to radioligand binding methods

Name: Additional file 2 of Design and validation of recombinant protein standards for quantitative Western blot analysis of cannabinoid CB1 receptor density in cell membranes: an alternative to radioligand binding methods
Creator: Borrega-Román, Leire; Saumell-Esnaola, Miquel; Barrondo, Sergio; González-Burguera, Imanol; Gómez-Caballero, Alberto; García del Caño, Gontzal; Elejaga-Jimeno, Ainhoa; Sallés, Joan; López de Jesús, Maider; Echeazarra, Leyre; Goicolea, María Aranzazu
Published: 2022-09-16 00:00:00
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Figshare2022-09-16 更新2026-04-08 收录

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https://springernature.figshare.com/articles/dataset/Additional_file_2_of_Design_and_validation_of_recombinant_protein_standards_for_quantitative_Western_blot_analysis_of_cannabinoid_CB1_receptor_density_in_cell_membranes_an_alternative_to_radioligand_binding_methods/21130053/1

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Additional file2: zip-compressed files of [i] theoretical sequences corresponding to pGEX-6P1 plasmids with DNA constructs inserted downstream the GST gene for the inducible expression of GST fusion proteins GST-CB1414-472 and GST-CB1414-442 and [ii] results of sequencing

附加文件2：为zip压缩格式文件，包含两项内容：[i] 对应pGEX-6P1质粒的理论序列，该质粒在谷胱甘肽S-转移酶（GST）基因下游插入了DNA构建体，用于诱导表达GST融合蛋白GST-CB1414-472与GST-CB1414-442；[ii] 测序结果

提供机构：

Borrega-Román, Leire; Saumell-Esnaola, Miquel; Barrondo, Sergio; González-Burguera, Imanol; Gómez-Caballero, Alberto; García del Caño, Gontzal; Elejaga-Jimeno, Ainhoa; Sallés, Joan; López de Jesús, Maider; Echeazarra, Leyre; Goicolea, María Aranzazu

创建时间：

2022-09-16