Transcriptional profiing of cultured adherent and non-adherent monocytes

To determine the effect of adherence on the metabolic and functional response of human monocytes. Monocytes were stimulated with lipopolysaccharide (LPS) under non-adherent and adherent conditions. To determine the role of glycolysis in LPS-induced immune responses, this pathway was inhibited by glucose deprivation or the glucose analog 2-deoxy-D-glucose (2DG). Monocytes were isolated from 6 healthy volunteers. Each donor's monocytes were exposed to every combination of the three treatment conditions (2 adherene conditions, 2 LPS conditions, 3 glucose conditions).

本研究旨在探究黏附状态对人单核细胞（human monocytes）代谢与功能应答的影响。实验中，单核细胞分别在非黏附与黏附两种条件下经脂多糖（lipopolysaccharide, LPS）刺激。为明确糖酵解在LPS诱导的免疫应答中的作用，本实验通过葡萄糖剥夺或葡萄糖类似物2-脱氧-D-葡萄糖（2-deoxy-D-glucose, 2DG）抑制该通路活性。单核细胞取自6名健康志愿者，每名供者的单核细胞均接受三种处理条件的全部组合：2种黏附条件、2种LPS处理条件及3种葡萄糖处理条件。