NEON Biorepository Soil Microbe Collection (Bulk Subsamples) (repackaging of occurrences published by the NEON Biorepository Data Portal)

This collection contains samples collected during periodic soil sampling and frozen at ultra-low temperatures in order to provide material for microbial sequencing or other microbial analyses (NEON sample classes: sls_soilCoreCollection_in.geneticArchiveSample1ID, sls_soilCoreCollection_in.geneticArchiveSample2ID, sls_soilCoreCollection_in.geneticArchiveSample3ID, sls_soilCoreCollection_in.geneticArchiveSample4ID, sls_soilCoreCollection_in.geneticArchiveSample5ID,sls_metagenomicsPooling_in.compositeSampleID). Archive samples are collected during each soil sampling bout and are promptly frozen. Three unique locations are sampled per plot, with ten plots per site. Bouts occur three times per year in order to capture the prevailing conditions at the site during different seasons, except in Alaska where only 1 bout is possible. Soil sampling is conducted to a maximum depth of 30 ± 1 cm, and when organic (O) and mineral (M) horizons are present within a single profile, they are separated prior to analysis and archiving. However, other sub-horizons are not separated. During the majority of bouts, only the top horizon (O if present, else M) is collected and archived. Soils are homogenized and non-soil material is removed by hand in the field, then subsamples are immediately frozen on dry ice. They are maintained in ultra-low temperature freezers until shipment to the Biorepository. See links below for NEON data products that provide physical, chemical, and biological measurements for these same soils (soil pH and moisture are always measured; chemical properties as well as microbial community composition and biomass are determined only for a subset of collection bouts). In addition, a more detailed characterization of the dominant soil types at each site, including taxonomy, texture, bulk density, and geochemical properties, occurred during the construction period of NEON through two projects. These data are available in NEON data products Soil physical and chemical properties, distributed initial characterization (DP1.10047.001) and Soil physical and chemical properties, Megapit (DP1.00096.001).

本数据集包含经定期土壤采样获取、并以超低温冷冻保存的样本，旨在为微生物测序或其他微生物分析提供实验材料（NEON样本分类：sls_soilCoreCollection_in.geneticArchiveSample1ID、sls_soilCoreCollection_in.geneticArchiveSample2ID、sls_soilCoreCollection_in.geneticArchiveSample3ID、sls_soilCoreCollection_in.geneticArchiveSample4ID、sls_soilCoreCollection_in.geneticArchiveSample5ID、sls_metagenomicsPooling_in.compositeSampleID）。每轮土壤采样都会获取存档样本，并即刻完成冷冻保存。每个样地设置3个独立采样点位，每个站点设10个样地。采样轮次每年开展3次，以捕捉不同季节站点的典型环境条件；阿拉斯加站点除外，该站点每年仅可开展1轮采样。土壤采样的最大深度为30±1 cm，若单个剖面同时存在有机层（O层）与矿质层（M层），则需在分析与存档前将二者分离，但无需区分其他亚层。多数采样轮次中，仅采集并存档表层土壤（若存在O层则采集O层，否则采集M层）。土壤样本需在野外经手工均质化处理，并去除非土壤杂质，随后将子样本即刻置于干冰中冷冻。样本将保存于超低温冰箱中，直至运送至生物样本库。可通过以下链接获取针对上述同款土壤的物理、化学与生物学测量数据（土壤pH值与含水率为必测指标；化学性质、微生物群落组成及生物量仅在部分采样轮次的样本中测定）。此外，在NEON建设阶段，通过两项项目完成了各站点优势土壤类型的详细表征，包括分类学、质地、容重及地球化学属性。相关数据可在NEON数据产品《土壤物理与化学性质：分布式初始表征（DP1.10047.001）》与《土壤物理与化学性质：大型探坑（DP1.00096.001）》中获取。