Transcription profiling of MamBo cell lines

We studied cell lines derived from two transgenic mammary tumors driven by human HER2 that showed different dynamics of HER2 status. MamBo89 (HER2 stable) cell line displayed high and stable HER2 expression, which was maintained upon in vivo passages, whereas MamBo43 (HER2 labile) cell line gave rise to HER2-negative tumors, from which MamBo38 (HER2 loss) cell line was derived. MamBo cell lines were established from mammary tumors of FVBhuHER2 virgin female mice. Transcription analysis was performed on the three cell lines with and without treatment with trastuzumab.

本研究针对两株由人表皮生长因子受体2（human HER2）驱动的转基因乳腺肿瘤来源的细胞系展开分析，这些细胞系的HER2状态呈现出不同的动态变化特征。其中MamBo89（HER2稳定型）细胞系可稳定高表达HER2，且该表达特征在体内传代过程中得以维持；而MamBo43（HER2不稳定型）细胞系可诱导产生HER2阴性肿瘤，由此进一步衍生出MamBo38（HER2缺失型）细胞系。本研究中的MamBo系列细胞系均源自FVBhuHER2品系未交配雌性小鼠的乳腺肿瘤。本研究针对上述三株细胞系，分别开展了曲妥珠单抗（trastuzumab）处理组与未处理组的转录分析。