DIS3L2 is an essential regulator of spermatogonial differentiation in mice

Using conditional knockout mice model, here we report the essential role of DIS3L2 in regulating male germline proliferation, growth and differentiation to ensure male fertility. We document that DIS3L2 is essential for spermatogonial homeostasis. DIS3L2-specific deletion in spermatogonia results in the reduction of the undifferentiated spermatogonial population and the block in transition to the differentiating stage in postnatal testes. RNA-seq was performed using postnatal day 4 (P4) testes between control and Dis3l2 cKO mice in order to identify the differentially expressed genes.

本研究借助条件性基因敲除（conditional knockout）小鼠模型，揭示了DIS3L2在调控雄性生殖系增殖、生长与分化以维持雄性生育能力方面的关键作用。研究证实，DIS3L2对精原细胞稳态至关重要；在精原细胞中特异性敲除DIS3L2，会导致出生后睾丸内未分化精原细胞群体减少，并阻断其向分化阶段的进程。为鉴定差异表达基因，本研究对对照组与Dis3l2条件性敲除（cKO）小鼠的出生后第4天（P4）睾丸样本进行了RNA测序（RNA-seq）。