Glutaminase-1 Inhibition Alleviates Senescence of Wharton’s Jelly-derived Mesenchymal Stem Cells via Senolysis.. Glutaminase-1 Inhibition Alleviates Senescence of Wharton’s Jelly-derived Mesenchymal Stem Cells via Senolysis.

we attempted to identify the genetic changes in the remaining MSCs after BPTES treatment and identified senescence-related candidate genes that recovered after BPTES treatment. The transcriptomes of U-MSCs and rS-MSCs were analyzed to determine the genetic significance of replicative senescence in WJ-MSCs Overall design: Comparative gene expression profiling analysis of RNA-seq data for U-MSCs and rS-MSCs with BPTES treatment Comparative gene expression profiling analysis of RNA-seq data for 3lots of U-MSCs and rS-MSCs

本研究旨在鉴定BPTES处理后留存的间充质干细胞（Mesenchymal Stem Cells, MSCs）的遗传变化，并筛选出经BPTES处理后表达恢复的衰老相关候选基因。通过分析U-MSCs与rS-MSCs的转录组，明确复制衰老在华通胶脐带间充质干细胞（Wharton's Jelly-Mesenchymal Stem Cells, WJ-MSCs）中的遗传调控意义。
实验设计总览：
1. 对经BPTES处理的U-MSCs与rS-MSCs的RNA测序（RNA-seq）数据开展比较基因表达谱分析；
2. 对3批U-MSCs与rS-MSCs的RNA测序（RNA-seq）数据开展比较基因表达谱分析。