Gene expression of healthy airway epithelium and p.Sdel560 STAT3-epithelium (Job Syndrome patient) in homeostasis and after 1 hour infection with pseudomonas aeruginosa

To explore the mechanism that underlies the lung infection susceptibility in job syndrome patients, we derived primary airway basal stem cells from a job syndrome patient which harbors a p.Sdel560-STAT3 mutation. After ALI differentiation, healthy airway epithelium and p.Sdel560 STAT3-epithelium were exposed to pseudomonas aeruginosa for 1 h. Then we collect the RNA to perform bulk-RNA sequencing. We then performed gene expression profiling analysis using data obtained from RNA-seq. Overall design: Comparative gene expression profiling analysis of RNA-seq data for healthy airway epithelium and p.Sdel560 STAT3-epithelium (Job Syndrome patient) in homeostasis and after 1 hour infection with pseudomonas aeruginosa.

为探究Job综合征（Job Syndrome）患者肺部感染易感性的潜在分子机制，本研究从携带p.Sdel560-STAT3突变的Job综合征患者体内分离得到原代气道基底干细胞，并以健康个体的原代气道基底干细胞作为对照。经气液界面（Air-Liquid Interface, ALI）分化培养后，将健康气道上皮细胞与携带p.Sdel560-STAT3突变的气道上皮细胞分别以铜绿假单胞菌（Pseudomonas aeruginosa）感染处理1小时，随后收集细胞总RNA开展批量RNA测序（bulk-RNA Sequencing）。基于获取的RNA测序（RNA-seq）数据进行基因表达谱分析。整体实验设计：针对稳态条件下及经铜绿假单胞菌感染1小时后的健康气道上皮细胞与携带p.Sdel560-STAT3突变的气道上皮细胞（源自Job综合征患者）的RNA测序数据，开展对比基因表达谱分析。