Additional file 5 of Antennal transcriptome analysis reveals sensory receptors potentially associated with host detection in the livestock pest Lucilia cuprina

Supplementary material 5: Table S4. Differentially expressed transcripts in the antennae of Lucilia cuprina. (A) Transcripts expressed at a significantly higher or lower level in attracted females relative to non-attracted females (AF vs. NF tab). (B) Transcripts expressed at a significantly higher or lower level in attracted females relative to males (AF vs M tab). (C) Transcripts expressed at a significantly higher or lower level in non-attracted females relative to males (NF vs M tab). DESeq2 was used to determine differentially expressed transcripts, using a P adjusted value < 0.05, and without fold-change (FC) threshold, being Log2FC < 0 considered downregulated and Log2FC > 0 upregulated. The number of down- and upregulated transcripts and genes (in parentheses), for each comparison is shown at the top left of each sheet. The differential expression is provided in Log2FC and percentage. Putative odorant degrading enzymes (ODEs) were noted in the last column of each table; (D) all differentially expressed transcripts (TPM > 0) were plotted using volcano plots as gray crosses. . Differentially expressed genes between the groups that are discussed in the manuscript are highlighted in bold.The X-axis and Y-axis represent Log2 fold-change (Log2FC) differences between the contrasted groups and statistical significance as the negative Log10 of the P adjusted value, respectively. Abbreviations: DOWN: downregulated, UP: upregulated, TPM: transcript per million, AF: attracted female antenna, NF: non-attracted female, M: male antenna.

补充材料5：表S4。铜绿蝇（Lucilia cuprina）触角中的差异表达转录本。(A) 相较于未吸引雌虫，吸引雌虫触角中表达量显著上调或下调的转录本（AF vs NF工作表）。(B) 相较于雄虫，吸引雌虫触角中表达量显著上调或下调的转录本（AF vs M工作表）。(C) 相较于雄虫，未吸引雌虫触角中表达量显著上调或下调的转录本（NF vs M工作表）。本研究采用DESeq2软件鉴定差异表达转录本，筛选条件为校正后P值（P adjusted value）<0.05，未设置倍数变化（fold-change, FC）阈值，其中Log2FC<0定义为下调，Log2FC>0定义为上调。各比较组的下调、上调转录本及基因（括号内为基因数量）的数量标注于每个工作表的左上角。差异表达数据以Log2FC及百分比形式呈现。各表格的最后一列标注了推定的气味降解酶（odorant degrading enzymes, ODEs）。(D) 所有满足TPM>0的差异表达转录本均以火山图绘制，以灰色叉点表示。手稿中讨论的组间差异表达基因以粗体标注。横轴代表对比组间的Log2倍数变化（Log2 fold-change, Log2FC），纵轴代表以校正后P值的负Log10值计算的统计学显著性。缩写说明：DOWN：下调，UP：上调，TPM：每百万转录本（transcript per million），AF：吸引雌虫触角，NF：未吸引雌虫，M：雄虫触角。