Comparison of different hashing methods on human PBMCs and primary mice tissues

the aim 1 was to evaluate hashing (sample multiplexing) accuracy of TotalSeq-B antibodies and CellPlex (a multiplexing solution from 10x Genomics) on 2 pools of PBMCs from 3 different donors. Thus the genetic difference between donors was used as a ground truth for sample demultiplexing based on antibody or lipid hashing hashing. the aim 2 was to evaluate TotalSeq antibody and lipid hashing on different mice primary tissues using different hashing protocols.

目标1：评估TotalSeq-B抗体（TotalSeq-B antibodies）与CellPlex（10x Genomics推出的多重化解决方案）在来自3名不同供者的2份外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMC）混合池中的哈希（样本多路复用）准确性。因此，本研究以供者间的遗传差异作为基于抗体或脂质哈希的样本解复用的金标准。目标2：采用不同的哈希实验方案，在多种小鼠原代组织中评估TotalSeq抗体与脂质哈希的性能。