CIL:20813, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

The membrane along the collecting canal of ruptured cells shows that the excess smooth spongiome membrane forms a three-dimensional array similar to etiolated chloroplasts when a piece of wood stays on a grass lawn too long preventing photosynthesis from occurring due to the lack of sunlight. Sometimes this configuration of membranes is referred to as cubic membranes (Landh, FEBS Lett. 369:15-17, 1995). TEM taken on 8/8/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5µm. Adapted with permission from the J. Cell Sci. 112:3733-3745, 1999. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:13116) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

破裂细胞收集管沿线的膜结构显示，过量的光滑海绵体膜（smooth spongiome membrane）会形成三维阵列，其形态类似因缺光黄化的叶绿体，此类场景常见于木板长期覆盖草坪、导致光合作用无法进行的情况。这类膜结构有时被称为立方膜（cubic membranes），相关研究见于Landh发表于《FEBS Letters》（FEBS Lett.）1995年第369卷第15-17页的文献。本透射电子显微镜（TEM）图像由R. Allen于1996年8月8日拍摄，使用蔡司10A型电镜（Zeiss 10A），加速电压为80kV，原始放大倍数12000倍，比例尺为0.5微米（µm）。本文图像经许可改编自1999年发表于《Journal of Cell Science》（J. Cell Sci.）第112卷第3733-3745页的研究。原始负片经冲印后扫描至Photoshop软件进行处理，本数字化图像可用于定性分析。该图像的未处理高分辨率版本（编号CIL:13116）馆藏于资料库中，可用于定量分析。样本制备采用标准流程：经戊二醛（glutaraldehyde）固定后，以四氧化锇（osmium tetroxide）染色，乙醇梯度脱水，最终包埋于环氧树脂（epoxy resin）中；切片由超薄切片机（microtome）制备，厚度约为75纳米（nm）。更多相关信息可访问网址：http://www5.pbrc.hawaii.edu/allen/