Effect of depletion of FABP4 in mice intestinal epithelial cells on gene expression

To investigate the function of FABP4 in the intestinal in vitro cultured organoids,FABP4, which located in crypt lyszyme+ Paneth cells can regulate the expression of defensins, especially HFD-mediated downregulation of defensin in Paneth cells will provide insights into factor(s) underlying modern diseases. We then performed gene expression profiling analysis using data obtained from RNA-seq of FABP4fl/flpvillinCreT (FABP4KO) mice and FABP4fl/fl (WT)mice in vitro cultured intestinal organoids. Overall design: Comparative gene expression profiling analysis of RNA-seq data for FABP4fl/flpvillinCreT (FABP4KO) mice and FABP4fl/fl (WT)mice in vitro cultured intestinal organoids.

为探究脂肪酸结合蛋白4（Fatty Acid Binding Protein 4, FABP4）在体外培养小肠类器官（intestinal organoids）中的功能，本研究聚焦于定位于隐窝溶菌酶阳性潘氏细胞的FABP4：该蛋白可调控防御素的表达，而高脂饮食（High-Fat Diet, HFD）介导的潘氏细胞防御素表达下调现象，将为阐明现代疾病的潜在致病因子提供重要研究视角。随后，我们利用FABP4fl/flpvillinCreT（FABP4敲除, FABP4KO）小鼠与FABP4fl/fl野生型（WT）小鼠的体外培养小肠类器官的RNA测序（RNA-seq）数据，开展基因表达谱分析。整体实验设计：针对FABP4fl/flpvillinCreT（FABP4KO）小鼠与FABP4fl/fl野生型（WT）小鼠的体外培养小肠类器官的RNA测序（RNA-seq）数据，进行比较性基因表达谱分析。