Antiviral innate immunity of hepatitis C virus-infected stem cell-derived hepatocytes. Antiviral innate immunity of hepatitis C virus-infected stem cell-derived hepatocytes

Purpose: RNAseq analysis of hPSC undergoing in vitro hepatic differentiation, to validate proper differentiation at different times of differentiation (D0 to D21) Overall design: Differentiated hepatocytes were then subjected to HCV infection (HCV compared to non infected NINF control), with or without various antiviral treatment (TEL) or JAK/STAT pathway inhibitor treatment (JSI), followed by analysis of global gene expression change. Particular emphasize was done on Innate Immune Genes and Interferon Regulated Genes (IRG) For each sample, individual run. hPSC undergoing in vitro hepatic differentiation. Differentiated hepatocytes subjected to HCV infection (HCV compared to non infected NINF control), with or without various antiviral treatment (TEL) or JAK/STAT pathway inhibitor treatment (JSI).

研究目的：对体外进行肝向分化的人多能干细胞（hPSC）开展RNA测序分析，以验证其在分化不同阶段（D0至D21）的分化是否正常。
实验整体设计：将诱导分化得到的肝细胞进行丙型肝炎病毒（HCV）感染实验，以未感染的NINF对照作为参照，并设置辅以各类抗病毒治疗（TEL）或JAK/STAT通路抑制剂治疗（JSI）的组别，随后对样本进行全局基因表达变化分析。本研究重点聚焦于先天免疫基因与干扰素调控基因（IRG）。
所有样本均单独进行测序上机。本实验的研究对象为体外诱导肝向分化的人多能干细胞，分化所得肝细胞接受HCV感染（以未感染的NINF对照为参照），并可辅以或不辅以各类抗病毒治疗（TEL）或JAK/STAT通路抑制剂治疗（JSI）。