Hepatic gene expression in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles. Mus musculus

We investigated hepatic mRNA profiles of female mice exposed to 0.162 mg Printex 90 carbon black nanoparticles by single intra-tracheal instillation. We examined responses to this dose 1, 3 and 28 days after exposure, alongside respective controls. We show that genes part of the 3-hydroxy-3-methyl-glutaryl-CoA reductase pathway to be up-regulated by exposure to Printex 90. Overall design: individual total RNA (200 ng) from 6 mice per treatment group (control and 0.162mg) and universal reference total RNA (Stratagene, Mississauga, ON, Canada) was used to synthesize double stranded cDNA.

本研究针对经单次气管内滴注暴露于0.162 mg Printex 90炭黑纳米颗粒的雌性小鼠，对其肝脏mRNA表达谱展开分析。我们分别于暴露后1天、3天及28天检测该剂量下的生物学应答，并同步设置对应对照组。本研究发现，暴露于Printex 90可使3-羟基-3-甲基戊二酰辅酶A还原酶通路（3-hydroxy-3-methyl-glutaryl-CoA reductase pathway）相关基因的表达水平上调。整体实验设计：每个处理组（对照组与0.162mg剂量组）各取6只小鼠的个体总RNA（200 ng），结合通用参考总RNA（Stratagene，加拿大安大略省密西沙加市），用于合成双链cDNA。