Transcriptome analysis of genes regulated by CUP-SHAPED COTYLEDON1 (CUC1) transcription factor in Arabidopsis thaliana

Samples 1 & 2: Comparison of gene expression between wild type (ecotype Ler) and 35S:CUC1, in which CUP-SHAPED COTYLEDON1, a master regulator of the shoot meristem and boundary establishment, is constitutively expressed under the cauliflower mosaic virus 35S promoter. Samples 3 & 4: Comparison of gene expression between stm-1 (ecotype Ler) and stm-1 35S:CUC1 RNA was isolated from cotyledons of 5-day-old (for Ler vs 35S:CUC1) or 10-day-old (for stm vs 35S:CUC1stm) seedlings. Two independent biological replicates were analyzed for each comparison.

样本1与样本2：野生型（生态型Ler）与35S:CUC1的基因表达比较；其中CUP-SHAPED COTYLEDON1（CUC1）作为茎尖分生组织与边界建立的主调控因子，在花椰菜花叶病毒35S启动子驱动下组成型表达。样本3与样本4：stm-1（生态型Ler）与stm-1 35S:CUC1的基因表达比较。RNA提取自5日龄（用于Ler与35S:CUC1对比组）或10日龄（用于stm与35S:CUC1stm对比组）幼苗的子叶。每组对比均设置两个独立的生物学重复进行分析。