Comparison of serological and molecular assays for Bartonella species in dogs with hemangiosarcoma

Currently, a gold standard diagnostic test for Bartonella infection in dogs is lacking. This represents a critical limitation for the development and evaluation of new diagnostic tests, as well as for the diagnosis of, and research on, bartonellosis in dogs. This retrospective observational study aims to compare the results of commonly performed and newly-reported Bartonella spp. diagnostic tests in banked clinical specimens from 90 dogs with hemangiosarcoma (HSA) using composite reference standard (CRS) and random effects latent class analysis (RE-LCA) techniques. Samples from each dog were tested using six serological or molecular diagnostic assays, including indirect fluorescent antibody (IFA) and Western blot (WB) for the detection of antibodies in serum, and qPCR and droplet digital PCR (ddPCR) in blood and fresh frozen tissue biopsy samples (mainly splenic HSA tumors and histopathologically normal spleen or skin/adipose tissue). Bartonella infection prevalence was estimated to be ...

目前，犬巴通体（Bartonella）感染缺乏金标准诊断试验。这对新型诊断试验的开发与评估，以及犬巴通体病（bartonellosis）的诊断与研究构成了关键限制。本回顾性观察研究旨在采用复合参考标准（CRS）与随机效应潜在类别分析（RE-LCA）技术，比较90例血管肉瘤（HSA）犬存档临床标本中常用及新报道的巴通体属（Bartonella spp.）诊断试验结果。每只犬的样本均通过6种血清学或分子诊断试验进行检测，包括用于血清抗体检测的间接荧光抗体（IFA）与蛋白质印迹（WB），以及用于血液和新鲜冷冻组织活检样本（主要为脾脏血管肉瘤肿瘤、组织病理学正常的脾脏或皮肤/脂肪组织）的定量聚合酶链式反应（qPCR）与微滴式数字聚合酶链式反应（ddPCR）。巴通体感染率估计为……