Peripheral blood whole transcriptome in patients with acute myocardial infarction and at follow-up

There is an unmet need to develop new strategies to improve risk stratification in patients with myocardial infarction and to identify new targets for intervention. The aim of this study was to establish the unbiased peripheral blood whole transcriptome response in myocardial infarction, and to correlate it with clinical characteristics and outcome. RNA expression was determined in the acute phase of MI and at follow-up, and related to clinical phenotype and outcome. We performed peripheral blood (PAXgene) RNA profiling in 192 samples: acute MI (n=65), 30 days post-MI (n=64), 1 year post-MI (n=37), patients with stable CAD (n=22) and technical replicates (n=4). The unbiased RNA profile was determined in acute MI, and we determined to potential of selected transcript to improve prediction of clinical outcome (eg. left ventricular dysfunction).

目前仍存在未被满足的临床需求，即开发新策略以改善心肌梗死（Myocardial Infarction, MI）患者的风险分层，并识别新的干预靶点。本研究旨在明确心肌梗死患者外周血全转录组的无偏倚应答特征，并将其与临床特征及预后相关联。研究检测了心肌梗死急性期及随访阶段的RNA表达水平，并分析其与临床表型及预后的关联。本研究共纳入192份样本进行外周血（PAXgene）RNA谱分析，包括急性心肌梗死样本65份、心肌梗死后30天样本64份、心肌梗死后1年样本37份、稳定性冠状动脉粥样硬化性心脏病（Coronary Artery Disease, CAD）患者样本22份，以及技术重复样本4份。本研究明确了急性心肌梗死患者的无偏倚RNA表达谱，并评估了筛选出的转录本用于改善临床结局（如左心室功能不全）预测的潜力。