miR-203 and miR-205 expression patterns identify subgroups of prognosis in cutaneous squamous cell carcinoma

Identification of the expression pattern of miRNAs at different stages of skin cancer progression in a panel of murine skin cancer cell lines miR-203 and miR-205 were differentially expressed in this panel, and were evaluated as biomarkers of prognosis in human cutaneous squamous cell carcinoma Three main tumor phenotypes: (i) Squamous non-malignant: Five non-malignant cell lines: three of them immortalized keratinocyte cell lines, and of them two papilloma cell lines. (ii) Three malignant squamous cell lines. (iii) Five malignant spindle cell lines. One replicate per array, except A5 that was done in duplicate as control

本研究针对一组小鼠皮肤癌细胞系，解析了皮肤癌进展不同阶段的微小RNA（miRNAs）表达谱。本研究队列中miR-203与miR-205呈差异表达，且二者已被评估为人类皮肤鳞状细胞癌的预后生物标志物。本数据集涵盖三类主要肿瘤表型：(1) 鳞状非恶性型：共5株非恶性细胞系，其中3株为永生化角质形成细胞系，2株为乳头状瘤细胞系；(2) 3株恶性鳞状细胞系；(3) 5株恶性梭形细胞系。除A5样本设置两次重复作为对照外，其余每个基因芯片仅设置1次重复。