Identification of LncRNA Expression Signatures for Triple-negative Breast Cancer. Homo sapiens

To identify biologically and clinically novel lncRNAs potentially involved in the progression of breast cancer, we profiled the expression of lncRNAs in two stage III triple-negativebreast cancer tissues and their paired adjacent noncancerous tissues by LncRNA Array 3.0 (ArrayStar). Expression of the mostly upregulated lncRNA (BCAR4) from this signature was quantified in the breast caner tissue microarray by RNA In situ Hybridization and bioinformatic analysis of Oncomine database, confirming its correlation with breast cancer metastasis. Overall design: LncRNA Expression profiling analysis of the 4 samples including 2 triple-negative breast cancer tissues and their matched adjacent normal breast tissues by Arraystar Human LncRNA Array v3.0.

为鉴定可能参与乳腺癌进展的兼具生物学与临床创新性的长链非编码RNA（long non-coding RNA，lncRNA），本研究采用Arraystar长链非编码RNA芯片3.0，对2例Ⅲ期三阴性乳腺癌组织及其配对的癌旁正常组织中的lncRNA表达谱进行检测。本研究通过RNA原位杂交（RNA In situ Hybridization）技术，对该表达特征中上调最显著的lncRNA（BCAR4）在乳腺癌组织芯片中的表达水平进行定量，并借助Oncomine数据库开展生物信息学分析，证实其与乳腺癌转移存在相关性。实验整体设计：采用Arraystar人类长链非编码RNA芯片v3.0，对4例样本（包含2例三阴性乳腺癌组织及其配对的癌旁正常乳腺组织）进行lncRNA表达谱分析。