m6A-seq profiling in Xist and Xist_Del_A-repeat cell lines

By m6A-seq, we examined the m6A pattern on Xist and Xist_Del_A-repeat, which immediately after the A-repeat region. A-repeat deletion abolished the m6A methylation downstream the A-repeat but not the m6A peak located in Xist exon VII. M6A profiling in cell lines with WT Xist or Xist lacking the A-repeat. We are aiming to understand how m6A pattern was on Xist and how m6A modification on Xist affect Xist-mediated chromosome silencing.

本研究通过m6A测序（m6A-seq）技术，检测了X染色体失活特异性转录本（Xist）及其A重复序列缺失突变体Xist_Del_A-repeat上的m6A修饰模式；该突变体的缺失区域紧邻A重复序列区域。实验结果显示，A重复序列的缺失会消除其下游区域的m6A甲基化修饰，但不会影响Xist第VII外显子上的m6A峰。此外，我们还对携带野生型（Wild Type, WT）Xist或A重复序列缺失型Xist的细胞系开展了m6A图谱分析。本研究旨在解析Xist上的m6A修饰模式，以及Xist的m6A修饰如何调控Xist介导的染色体沉默过程。