GSE47296: JHDM1_Q2634.14-16 hr OR embryo.Solexa

Summary: modENCODE_submission_5114 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using the Illumina NGS platform. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: Embryo 14-16hr OR; Genotype: wild type; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Antibody JHDM1 Q2634 (target is JHDM1); Developmental Stage Embryo 14-16hr OR

数据集摘要：modENCODE_submission_5114
本提交数据来自Gary Karpen主持的modENCODE项目。如需查看modENCODE项目完整列表，请访问http://www.genome.gov/26524648。
项目目标：本研究旨在定位黑腹果蝇（Drosophila melanogaster）全基因组范围内的125种染色体蛋白。本次研究的蛋白参与DNA复制、基因表达、基因沉默及遗传等核心染色体生物学功能。本研究将依托Illumina二代测序（Next Generation Sequencing，NGS）平台开展染色质免疫共沉淀（Chromatin ImmunoPrecipitation，ChIP）实验。研究初期将使用3种细胞系和2个胚胎时期的染色质进行蛋白定位检测，后续将把部分蛋白的分析范围拓展至另外4种动物组织/发育时期。有关数据使用条款与规范，请参阅http://www.genome.gov/27528022及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
实验设计总览：
实验类型：ChIP-seq
生物样本来源：品系：Oregon-R（官方命名：Oregon-R-modENCODE，基因型：野生型）；发育时期：胚胎14-16小时（OR品系）；基因型：野生型
实验变量：品系：Oregon-R（官方命名：Oregon-R-modENCODE，基因型：野生型）；抗体：JHDM1 Q2634（靶蛋白为JHDM1）；发育时期：胚胎14-16小时（OR品系）