CIL:9920

Image of the extracellular, non-contractile stalk of Opercularia coarctata that is formed from the scopula. Cylinders ('straws') of fibrous material in the lumen of the stalk are surrounded by an outer membrane-like sheath. The fibrous cylinders have diameters similar to those of basal bodies. TEM taken on 6/10/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:7455) is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

本图像展示缢缩盖虫（Opercularia coarctata）由刷状器（scopula）形成的细胞外非收缩柄结构。该柄内腔中的纤维性圆柱结构（俗称“吸管”）被一层类外膜鞘包裹。此类纤维圆柱的直径与基体（basal body）相近。
本透射电子显微镜（Transmission Electron Microscopy, TEM）图像由R. Allen于1969年6月10日拍摄，采用加速电压为60kV的飞利浦（Philips）300型电镜，放大倍数为14800倍，标尺为0.5μm。底片冲印件经扫描后在Photoshop中进行后期处理。本图像仅适用于定性分析，如需进行定量分析，可使用高分辨率图像（CIL:7455）。
样品制备遵循标准流程：先经戊二醛固定，再以四氧化锇固定，随后经酒精脱水、环氧树脂包埋，最终通过超薄切片机制备厚度约75nm的切片。更多相关信息可访问：http://www5.pbrc.hawaii.edu/allen/。