Equine RNAseq Tissue pool

This dataset is comprised of RNA samples from 11 horses of various ages and sex that comprise the following tissues: Adipose Tissue, Adrenal Cortex, Adrenal Medulla, Aorta, Articular Cartilage (foal), Bladder, Bone, Bone Marrow, Cecum, Cerebrum, Cornea, Embryo (whole embryo, 34d), Endometrium (preg. day 16), Endometrium (preg. day 50), Epididymus, Hoof (germinal epithelium), Kidney, Large Intestine, Liver, Lung Lymph Node, Lymphocytes (activated), Muscle (cardiac), Muscle (skeletal, tongue), Muscle (skeletal), Muscle (smooth), Ovary, Pancreas, Pituitary (anterior), Pituitary (posterior), Placental Villous, Retina, Salivary Gland, Skin (full thickness), Spinal Cord, Spinal Root Ganglia, Spleen (red pulp), Spleen (white pulp), Stomach, Synovial Membrane, Tendon (superficial digital flexor, foal), Testes, Thymus, and Vena Cava. This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. Tissue samples for total RNA isolation were collected under a protocol (00814A2004) reviewed and approved by the Office of Research Integrity, Institutional Animal Care and Use Committee, at the University of Kentucky (PHS Assurance Number: A3336-01). Two micrograms of mRNA from each tissue sample was used to generate the final RNA pool. The pooled sample was Illumina and 454 sequenced with the aim to improve equine gene structural annotation.

本数据集包含11头不同年龄、不同性别的马的RNA样本，涵盖以下组织：脂肪组织、肾上腺皮质、肾上腺髓质、主动脉、幼驹关节软骨、膀胱、骨、骨髓、盲肠、大脑、角膜、胚胎（完整胚胎，妊娠34天）、子宫内膜（妊娠第16天）、子宫内膜（妊娠第50天）、附睾、蹄（生发上皮）、肾脏、大肠、肝脏、肺淋巴结、活化淋巴细胞、心肌、骨骼肌（舌肌）、骨骼肌、平滑肌、卵巢、胰腺、垂体前叶、垂体后叶、胎盘绒毛组织、视网膜、唾液腺、全层皮肤、脊髓、脊神经节、脾脏红髓、脾脏白髓、胃、滑膜、幼驹指浅屈肌腱、睾丸、胸腺、腔静脉。本研究严格遵循美国国立卫生研究院（National Institutes of Health, NIH）《实验动物护理与使用指南》中的相关建议开展。用于总RNA提取的组织样本，采集自肯塔基大学（University of Kentucky）研究诚信办公室与机构动物护理和使用委员会审查并批准的实验方案（编号：00814A2004），该机构的公共卫生服务（Public Health Service, PHS）保证编号为A3336-01。每份组织样本取2微克mRNA用于构建最终的RNA混合样本池。对该混合样本进行Illumina与454双平台测序，旨在完善马属动物基因结构注释。