Expression in kiwifruit under drought stress

In this study, 18 kiwifruit seedlings of uniform size were selected as experimental materials and divided into two groups of 9 plants each. 9 plants were used as the control group with soil moisture maintained at 80-85% and then divided into three groups, numbered CK1, CK2 and CK3; the other 9 plants were subjected to a drought treatment, in which the watering was stopped until the soil humidity was 40-45%, and they were subjected to drought stress for 72 h and randomly divided into three groups, numbered T1, T2 and T3. 3 samples in each numbered group were observed and recorded, and physiological indexes such as plant height and leaf relative water content were observed and recorded sequentially. The other 9 plants were randomly divided into three groups, numbered as T1, T2 and T3. 3 samples were recorded in each group, and the growth indexes such as plant height and leaf relative water content were observed and recorded in each group, and the leaves of each sample were taken out as experimental materials in turn, and stored in liquid nitrogen for transcriptome sequencing.

本研究选取18株规格均一的猕猴桃实生苗作为试验材料，将其分为两组，每组各9株。其中9株作为对照组，维持土壤含水量为80%~85%，随后将该对照组划分为三组，编号依次为CK1、CK2、CK3；剩余9株接受干旱处理，停止浇水直至土壤湿度降至40%~45%，使其经受72小时干旱胁迫，随后随机分为三组，编号为T1、T2、T3。每组选取3个样本进行观测记录，依次测定株高、叶片相对含水量等生理指标。该干旱处理组随机分为三组，编号为T1、T2、T3，每组均记录3个样本，观测并记录株高、叶片相对含水量等生长指标，随后依次采集每个样本的叶片作为试验材料，置于液氮（liquid nitrogen）中保存，用于转录组测序（transcriptome sequencing）。