Agaricus bisporus respiratory bursts

Respiratory bursts were observed in A. bisporus. CO2 production and O2 consumption increased up to 3.5 fold during these 3 h bursts while compost temperature increased up to 3 °C. We set out to find which pathways were characteristic for these bursts and the interval between these bursts. Total RNA expression by Agaricus bisporus production strain A15, was analyzed during respiratory bursts, after a burst and in the interval between two bursts (i.e. ~0.5 h, 4.5 h, and 8.5 h after a burst started, respectively), by Illumina sequencing. In total 9 samples were sequenced, each sample point of a burst having each 3 replicates.

在双孢蘑菇（Agaricus bisporus）中观察到呼吸爆发现象。在该3小时的呼吸爆发过程中，二氧化碳生成量与氧气消耗量最高可增至原水平的3.5倍，同时堆肥温度最高升高3℃。本研究旨在明确与该呼吸爆发及爆发间隔期相关的特征代谢通路。本研究采用Illumina测序技术，对双孢蘑菇生产菌株A15的总RNA表达量开展分析，采样时点分别覆盖呼吸爆发期、爆发后时期以及两次爆发间的间隔期（即呼吸爆发开始后约0.5 h、4.5 h与8.5 h）。本次测序共包含9个样本，每个采样时点均设置3次生物学重复。